Oncorhynchus mykiss

As an intermediate stage of complexity, fish embryos will be used as a tool to mediate the transfer of data compiled at the molecular and cellular levels (WPs 3 and 4) to intact, fully developed fish (WP 6). To this end, similar endpoints as in WP 4 will be recorded in modified zebrafish embryo assays [85]. In addition, extension of the test duration will allow adding of teratogenic effects to the list of endpoints.

The purpose of WP 4 is to provide a set of toxicological base data on the test substances at the mo-lecular and cellular levels. The focus will not be restricted to basic acute toxicity, but will also cover specific endpoints such as interaction with DNA and DNA damage, the induction of cytochrome P450 as well as the response to glucocorticoid stress hormones and metabolic signaling by glucose. With respect to the selection of test compounds, WP 4 depends on the outcome of WPs 1 and 2.